Components of compound microscope2/2/2023 ![]() ![]() Invented by Fritz Zernike, they convert small phase shifts in the light passing through the specimen into changes in contrast. Phase Contrast microscopes enable greater contrast in transparent specimens (protozoa etc) without the use of stains. They are designed for opaque or polished metal specimens that require high magnifications, but with reflected illumination (more typical in a stereo microscope). Metallurgical microscopes are a form of inverted microscope. The objectives are located below the stage while the light source and condenser are above the stage. They are also used for polished metal specimens where reflected light is required. ![]() ![]() For example, specimens in containers such as petri dishes. As a result, the specimen is illuminated against a dark background.Įpi-Fluorescence microscopes use the phenomena of fluorescent and phosphorescent light instead of, or in addition to, reflection and absorption.Īre used to view specimens that require more working space than a slide. They use scattered light that is not collected by the objective lens and so the light will not form part of the image. ![]() Brightfield microscopes use transmitted (illuminated from below) white light that is absorbed by denser (darker) areas of the specimen to create contrast.ĭarkfield microscopes improve the contrast in unstained, transparent specimens. ![]()
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